This is a Spider-Man ultimate event of the Year, Spider-Man must face the imposter Spider-Man's and reveal he is the true Spider-Man.
Spider-Man must deal with Spectacular, Amazing, Ultimate, and Superior Spider-Man and face archfoes in the way. Scott Bakula acts as the Spider-Man whom was made by Stan Lee long ago in the 60's!
Spider-Man/ Peter Parker (Jason Marsden) - with a costume from the Live Action Spider-Man, that Toby McGuire wore, and actual webbing.
Spectacular Spider-Man (Josh Keaton) - wears the similar costume as the Cartoon Spectacular Spider-Man, tells Peter that he is the true Spider-Man and bids him good luck
Amazing Spider-Man (Drake Bell) - dressed like the Amazing Spider-Man movie version, breaks the 4th wall by saying, "I still a 4-film franchise left, so good luck!, Spider-Man!"
Superior Spider-Man (Christopher Daniel Barnes) - is more like the Comics, The Superior Spider-Man.
Ultimate Spider-Man (Neil Patrick Harris) - more like the Ultimate Spider-Man, already believes that he is dead and Miles Morales is his successor
60's Spider-Man (Scott Bakula) - the classic Spider-Man, and the one that helped Spider-Man find the truth about "Great Power comes Great Responsibility"!
Mary Jane Watson (Emmanule Chirqui) - relizes that Peter is the true Spider-Man and the tiger she knows
Gwen Stacey (Alysa Millano) - girlfriend of the Amazing Spider-Man
J. Jonah Jameson (Wade Williams) - accuses Spider-Man as a clone and believes one of the Spider-Man to be the true Spider-Man
Robbie Robertson (Dwayne Johnson)
May Parker (Tabitha St. Germain)
George Stacey (Steven Blum) - used to think the Amazing Spider-Man was a masked vigilante, but now has heart towards him
Dr. Curt Connors (Rhys Ifans)
Harry Osborn (Michael Sinterniklass)
Steve Rogers/ Captain America (Rino Romano)
Tony Stark/ Iron Man (Will Friedle)
Ben Grimm/ The Thing (Clancy Brown)
Julia Carpenter/ Spider-Woman (Emmanule Chirqui)
Norman Osborn/ Green Goblin (Kevin Michael Richardson)
Otto Octavius/ Dr. Octopus (Corey Burton) - has been vanished along with his Superior Spider-Man into Mephisto's realm
Flint Marko/ Sandman (Wade Williams)
The Lizard (Rhys Ifans)
Alex O'Hirn/ Rhino (Ray Winestone)
Maxwell Dillon/ Electro (Crispin Glover)
Quinton Beck/ Mysterio (Stacey Keach)
Mac Gargen/ Scorpion (Michael Emmerson)
Adrian Toomes/ Vulture (Jeff Bennett)
Kraven the Hunter (Hector Elizondo)
Chameleon (Andre Soliquizzo)
Shocker (C. Thomas Howell)
Jason Macendale/ Hobgoblin (Mandy Patinkin)
Eddie Brock/ Venom (Travis J. Gould)
Cletus Cassidy/ Carnage (Greg Cipes)
Kaine (Neil Patrick Harris) - main antagonist of the whole Movie.
Dr. Miles Warren (W. Morgan Shepard)