In a sequel to Scooby-Doo and the Ghoul School, Shaggy and Scooby are called again, when the ghoul school girls are now teens, and Dracula warns Shaggy and Scooby that if Sibella falls in love with one of their kind, it will be a "bat" day for them, but when a human arrives, and Sibella falls in love with the human, Shaggy and Scooby prevent Dracula from knowing the truth. And then Ursula offers something deadly towards Sibella if she wants to marry the human, and it's worse when she was brought back to life by Hades, along with Jafar who plans to steal back his power.
- Scooby-Doo (Frank Welker)
- Shaggy Rogers (Matthew Lillard) - gets turned into a werewolf again.
- Fred Jones (Frank Welker)
- Daphne Blake (Grey DeLisle) - gets turned into Bimbo by Jafar.
- Velma Dinkley (Mindy Cohn)
- Lloyd Phillips (Jesse McCartney) - a 19-year old human who falls in love with Sibella, though Shaggy and Scooby dress him to look like Elsa's cousin
- Sibella Dracula (Grey DeLisle) - 16 years old, now falling in love with a human named Lloyd
- Tanis (E.G. Dailey) - 11 years old
- Elsa Frankenteen (Pat Musick) - 17-years old
- Phantasma (Kath Soucie) - 14-years old
- Winnie the Werewolf (Russi Taylor) - 12-years old
- Miss Grimwood (Glynis Johns)
- Matches (Frank Welker)
- Aladdin (Scott Weigner)
- Jasmine (Linda Larkin)
- Abu (Frank Welker)
- Genie (Robin Williams)
- Hercules (Tate Donovan)
- Phil (Danny Devito)
- Pegasus (Frank Welker)
- Jafar (Jonathan Freeman) - appears as the main antagonist, and gets imprisoned by Miss Grimwood.
- Ursula (Pat Carroll) - offers Sibella a chance to become human in exchange for her immortality
- Hades (James Woods) - appears as the second main antagonist, as he was the one who revived Jafar and Ursula
- Count Dracula (Corey Burton) - warns Shaggy and Scooby what would happen if Sibella is dating a human, then it would be a bat day for them
- This is based on the 2012 film, Hotel Transylvania.
- A Mixture of Disney movies such as Aladdin and Hercules!
- You're Only Second Rate - sung by Jafar, when he attacks the gang.
- Poor Unfortunate Souls - sung by Ursula, when tempting Sibella despite Shaggy and Scooby's attempts to stop the deception.
- Everybody has too Sleep - sung by Fred to tell Lloyd why sleep is important