Scooby and the gang visit their old friend, Kitty Periwinkle, who is hanging with the gang while they are at Old LeRange, but when various twisters start attacking the town, the Gang must find a way to escape it, by solving the mystery on who caused the storms.
- Scooby-Doo (Frank Welker)
- Shaggy Rogers (Matthew Lillard)
- Fred Jones (Frank Welker)
- Daphne Blake (Grey DeLisle)
- Velma Dinkley (Mindy Cohn)
- Kitty Periwinkle (Emma Stone) - the Gang's old friend
- Erika Periwinkle (Susan Roman) - Kitty's mother
- Ronald Periwinkle (Don Francks) - Kitty's father
- Bryce Jones (Andrew Garfield) - Kitty's love interest
- Helga Brand (Kath Soucie) - Kitty's friend who she hangs around with due to their parents being neighbors with each other
- The Tornado Monster Creator (Yuri Lowenthal) - the main antagonist, matches Albedo's Evil Way Big's tone, from Ben 10: Ultimate Alien: Cosmic Destruction
- Brian Ground (Ryan Gosling) - Kitty's jealous boyfriend, when she is hanging around with Bryce, Reason: well Scooby's suspician!
- Joyce Henson (Cathrine O'Hara) - the Mayor, Reason: Velma was getting to the bottom of her relection day!
- Lucas and Madison Brand (Kirk Thornton and Mary Elizebeth McGlynn) - Kitty's neighbors, whom their daughter, Helga hangs around with, Reason: Daphene seen how they look at Kitty!
- Sheriff Proctor Williams (Daniel Riordan) - Reason: Fred senses that he has a past with Ronald!
- Deputy Otis McLean (Neil Kaplan) - Reason: Fred sees him destroying the cloth evidence!
- Jack and Jake (Greg Cipes and Danny Cooksey) - 2 punks who are furiously jealous of Shaggy and Scooby hanging with their goth girls, Angel and Annie as they attacked them by wrecking the Mystery Machine, Reason: Shaggy and Scooby sees the same damage caused by the Tornado Monster
- Angel and Annie (Ashley Johnson and Catlyn Taylor Love) - 2 goth girls who are the girlfriends of Jack and Jake, Annie is bald, with a mohawk, and piercings, while Angel is raven haired, with a ponytail, Reason: Daphne and Velma senses they cause trouble for Shaggy and Scooby!
- Deputy Otis McLean (Neil Kaplan) - Reason: to make sure he replaces Proctor and gain the authority over the whole Town of Old LeRange!
- Joyce Henson (Catherine O'Hara) - Reason: to win the election, and become Governor!
- Angel and Annie (Ashley Johnson and Caitlyn Taylor Love) - Reason: they were hired by McLean!
Kitty Periwinkle and Bryce Jones are seen in Bryce's car, as they are listening to music, then suddenly a large hurricane tries to hit them, and it was caused by a creature known as the Tornado Monster Creator, who warns them to stay off his land.
Meanwhile, Scooby-Doo and Shaggy are being chased by the One-Eyed Monster, as Fred traps it in it's trap, and it was none other than ???, who wanted to ???, and stated that he would've gotten away with it too if it wasn't for those meddiling kids, then Fred, Daphne, Velma, Shaggy and Scooby take off in the Mystery Machine to visit Kitty Periwinkle, which was revealed that she was an old friend. Then they stopped by to meet Kitty at last, she hugs Daphne and Velma, they stare at her parents on how upset they were, her dad, Ronald was talking with Sheriff Proctor Williams. Then the gang came up to ask what happened, Proctor informed them that a tornado has hit the town. Helga Brand, Kitty's friend then came by and said hi to her, though her parents made a look at them.