- Ray Romano as Manny
- John Leguizamo as Sid
- Denis Leary as Diego
- Queen Latifah as Ellie
- Jennifer Lopez as Shira
- Keke Palmer as Peaches
- Josh Peck as Crash and Eddie
- Josh Gad as Louis
- Chris Wedge as Scrat
- Jay Leno as Fast Tony
- Josh Hutcherson as Roshan/Nunar
- Wanda Skyes as Granny
she is a New Zealand Pidgeon and Nagata's love intrest that lives in trees and will help Manny, Sid and Diego, to save their home from humans. She is voiced by Sandra Bullock.
he's a Irish Elk that helps, Manny, Sid and Diego save their habitat, he is voiced by Tom Kenny.
he is a Grey Mammoth, in the end, turns Peaches's real love interest. he's voiced by Nick Robinson.
a female Molehog and Louis's love interest. she's voiced by Emma Watson.
a Short Faced Bear He says that humans have destroyed your home and your family and you want revenge, in the end he joins the herd, similar to Boog from Open Season, he's voiced by Tyler Perry.
Lola Leona. She is voiced by Debby Ryan
The main antagonist, He is the uncle of Roshan, hates animals, he is the leader of the flock and plans to destroy the entire island. in the end, Manny throws it in the sea and he is killed by piranhas.
The secundary antagonist, he's a wolf hunting from Saw, it has the same tone of Alpha from Up. he is voiced by Jeff Bennett.
Otto and RarkEdit
They are wolves hunting of the other hunters, are idiots and they have the same tone of Beta and Gamma from Up.
A pre-historic Shark who chases Scrat in the middle of the film, is voiced by Chris Wedge.
A Mammoth who has a crush on Jay, enemy of Peaches, is voiced by Miranda Cosgrove
second of command of the tribe of Saw, in the end, reforms and accepts animals in Ice Age, voiced by Zachary Levi.